Protein Modification

Affiliate University: 
Radboud University
Main lecturer(s): 
Prof. Dr. G. Pruijn

Proteins are modified in several ways after ribosomal translation. Post-translational modifications include trimming, splicing, phosphorylation, glycosylation, oxidation, addition of membrane anchors, fusion with other proteins, alkylation, acetylation, and many other types of modification. As a result of such modifications, the potential diversity of the proteome, the complete collection of all proteins in a cell, tissue or organism, greatly exceeds the number of genes in the genome. Proteomes vary considerably between different cell types and tissue samples from the same organism. Chemical biology seeks to understand such differences.

In this course several types of post-translational modifications, methods to study such modifications and technologies to manipulate proteins based upon such modifications will be introduced.


  • Introduction to post-translational modifications
  • Glycosylation
  • Protein modification in epigenetic regulation
  • Protein splicing
  • Manipulation of protein expression levels (degrons)
  • Fusion proteins and detection